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Mycoplasma hominis aerobic or anaerobic

The presence of virulent bacteria (group A and B streptococci, aerobic Gram-negative rods, Neisseria gonorrhoeae, organisms associated with bacterial vaginosis or Mycoplasma hominis) increases the risk of endometritis, 30 as do prolonged rupture of membranes and multiple vaginal examinations mycoplasmic protein and arginine deiminase were determined for M. hominis under aerobic and anaerobic conditions, aerobically grown cells exhibited no detectable enzymatic increases until late in log phase. Higher levels of arginine deiminase were observed earlier in the anaerobic growth cycle. The rate of 14CO2 evolutio Mycoplasma are aerobic or facultative anaerobic microorganisms, but they usually grow better in an aerobic environment. The best culture environment for initial isolation is atmospheric conditions supplemented with 5% CO 2 or anaerobic conditions with 5% CO 2 and 95% N 2 M. hominis is a pathogen in humans commenly found as part of urogenital tract flora especially of women and sexually active adult males. This bacteria cause a variety of infections which may lead to pelvic inflammatory disease, post-abortal fever, post partum fever and extragenital infections for immunodepressed humans The AF was cultured for Mycoplasma hominis and Ureaplasma urealyticum as well as for aerobic and anaerobic bacteria. Eighteen (35%) patients with IAI and four (8%) controls had M. hominis in AF (P less than 0.001). Twenty-six (50%) patients with IAI and 26 (50%) control patients had U. urealyticum in AF (difference not significant)

For the metronidazole susceptible isolates under anaerobic and aerobic incubation, a larger proportion of these isolates (81.8% and 89.5%, respectively) harboured M. hominis when compared with the isolates who tested negative for the presence of M. hominis (Tables 2 and 3) Mycoplasmas are ubiquitous pathogens found not only in humans but also in animals, plants, insects and soil. Though they usually grow better in an aerobic environment, mycoplasmas are also facultative anaerobic microorganisms The mycoplasmas are facultative anaerobes, except for M. pneumoniae, which is a strict aerobe. A characteristic feature that distinguishes the mycoplasmas from other bacteria is the lack of a cell wall. Thus, they can assume multiple shapes including round, pear shaped and even filamentous

Mycoplasma hominis - an overview ScienceDirect Topic

a genus of highly pleomorphic, gram-negative, aerobic or facultatively anaerobic bacteria that lack cell walls, including the pleuropneumonia-like organisms and other species. Mycoplasma ho´minis a species found associated with nongonococcal urethritis and mild pharyngitis Start studying Aerobic Gram-Positive Rods, Spirochetes, Mycoplasmas and Ureaplasmas, and Chlamydia, Anaerobic Bacteria. Learn vocabulary, terms, and more with flashcards, games, and other study tools The specimens obtained after prostatic massage of the remaining 26 patients were completely negative for both aerobic and anaerobic bacteria. No Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum or Trichomonas vaginalis were isolated from these patients The inoculated medium was incubated for 48 h at 35°C in an anaerobic jar (GasPak Plus; Becton Dickinson Microbiology Systems, Cockeysville, MD). Fried-egg-appearing colonies were seen that were arginine-positive. The organism was identified as M. hominis. Anaerobic bacterial cultures were negative. All blood cultures remained negative

Only aerobic bacteria 0 Only anaerobic bacteria 18 Aerobic and anaerobic bacteria 6 Mycoplasma hominis 0 Ureaplasma ureolyticum 0 Chlamydia trachomatis 0 Negative 26 164 I. Szo¨ke et al. Ó 1998 Blackwell Science Ltd, International Journal of Andrology, 21, 163-16 Culturing Mycoplasma spp. is laborious, usually requiring special growth media, and, depending on species, can take up to several weeks [ 11, 12]. M. hominis is the only pathogenic Mycoplasma of human origin that can grow on routinely used bacteriologic blood-based media in both aerobic and anaerobic conditions and for which growth is usually ob Aerobic and Anaerobic Bacterial Flora in Semen from Fertile and Infertile Groups of Men M. S. E. REHEWY, E. S. E. HAFEZ, Mycoplasma hominis Ureaplasma urealyticum Total isolates 8 4 - 2 5 25 32 16 - - 8 20 100 16 11 19 13 3 2 3 2 4 3 30 20 7 5 5 4 6 4 1 4 3 3 2 3 2 10 7 10 7 21 14. A Mycoplasma hominis polymerase chain reaction (PCR) performed on the synovial fluid was negative, as were fungal and aerobic and anaerobic bacterial cultures. A computed tomographic scan revealed a mild left lower lobe lung infiltrate and mild right axillary lymphadenopathy

Mycoplasma - www

Role of arginine deiminase in growth of Mycoplasma hominis

  1. is. Free gas in the tissues, abscess formation and foul-smelling discharge is commonly associated with the presence of anaerobic bacteria
  2. antly Bacteroides species
  3. e) The bacteria are aerobic/facultative anaerobic f) Do not consist of flagella or spores 3) A 14-year-old boy contracts cough and fever at school where one of his friends from the class had the same symptom for a few days

Polymicrobial aerobic-anaerobic female genital tract infections include vulvovaginitis, vulvovaginal pyogenic infections Trichomonas vaginalis, Chlamydia trachomatis, herpes simplex, Mycoplasma hominis and Condyloma accuminata. Bacterial vaginosis is a synergistic infection caused by a complex alteration in the microbial flora,. Of the 50 patients investigated, in samples from 18 patients only anaerobic bacteria were found in >10 6 CFU/mL, while in six patients one aerobic and three or more anaerobic bacteria could be cultured (Table 1). None of the patients gave positive cultures for Mycoplasma hominis, Ureaplasma urealyticum or Chlamydia trachomatis Transport media should preserve the viability and stability of microorganisms in clinical specimens. In this study, the Port-A-Cul transport system and the Copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. Dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples

Anaerobic gram-negative rods and mycoplasmal bacteria, including Mycoplasma hominis and Ureaplasma urealyticum, have been isolated from the upper genital tract in women with endometritis and salpingitis [8, 10-14, 16-28] Mycoplasma pneumoniaeis one of the few species ofMycoplasmathat frequently cause infection in humans.M. pneumoniaepredominantly causes respiratory tract infecti Mycoplasma genitalium, Mycoplasma hominis, Although it grows under both aerobic and anaerobic conditions and can be isolated on media supplemented with serum, it is a fastidious. However, at 24°C, there was a significantly greater loss of viability for Mycoplasma hominis, Prevotella bivia, Prevotella corporis, and Peptoniphilus asaccharolyticus when the organisms were stored in Copan transport medium than when they were stored in Port-A-Cul transport medium for 96 h (P < 0.002) Mycoplasma hominis and Vaginal Dysbiosis. The bacterium Mycoplasma hominis is an obligate parasite of the human urogenital tract belonging to the class Mollicutes and is characterized by the absence of a rigid cell wall and by one of smallest genomes among self-replicating organisms (Taylor-Robinson, 2017) The point mutation results in aerobic resistance from damaged oxygen obtaining conduits [24]. The endosymbiotic relationship arises from T. vaginalis providing M. hominis with protection from environmental pressures that would normally kill M. hominis [24]. Mycoplasma hominis Infection Statistics As of September 2012, it is estimated that.

Mycoplasma - an overview ScienceDirect Topic

Immune dysfunction is a recognized feature of end-stage renal disease and is increased with immunosuppressive drugs following transplantation. Uncommon microorganisms usually of low virulence may become major pathogens in this clinical setting. We report the successful treatment of Mycoplasma hominis infection of a hematoma post-renal transplant nephrectomy, with ciprofloxacin monotherapy b) Mycoplasma pneumoniae c) Mycoplasma hominis d) Mycoplasma genitalium 7) Select the primary pathogens of genital infections a) Mycoplasma pneumoniae b) Mycoplasma hominis c) Ureaplasma urealyticum d) All of the above 8) The routine laboratory diagnostics method is usually not helpful for the isolation and identification of Mycoplasma pneumoniae It also discusses the role of anaerobic bacteria in tonsillitis and its complications. Aerobic Bacteria. Mycoplasma pneumoniae and Mycoplasma hominis can cause PT usually as a manifestation of a generalized infection. The prevalence of Mycoplasma infection increases with age Mycoplasma hominis and Ureaplasma spp. are rare causes of brain abscess in neonates, and there are no detailed reports in the medical literature of previous cases. We suspect that our patient developed a mycoplasmal and ureaplasmal CSF infection that evolved into a brain abscess Aerobic Gram negative bacilli - nonfermenters Anaerobic Gram negative bacilli Burkholderia spp Coxiella burnetii Ehrlichia spp Enterobacteriaceae Enterococcus spp Listeria monocytogenes. Metamycoplasma hominis (previously Mycoplasma hominis) Pseudomnonas aeruginosa Pseudomonas spp Staphylococcus aureus (MRSA) Stenotrophomonas maltophili

Although aerobic and anaerobic isolates were frequently identified before treatment, no comparisons of microbiologic cure for these pathogens were presented. Mycoplasma hominis, Mycoplasma. > 72 h @ 25 ± 1°C, aerobic > 72 h @ 36 ± 1°C, aerobic 3. Biological control Inoculum size for productivity: 10 2 - 10 3 cfu per tube Inoculum size for selectivity: 10 3 - 10 4 cfu per tube Incubation conditions: 48 h @ 36 ± 1°C, anaerobic Control Strain Growth Mycoplasma hominis ATCC ® 14027 Ureaplasma urealyticum ATCC ® 2761 Bacterial vaginosis (BV) is caused by an overgrowth of anaerobic bacteria in the vagina, leading to depletion of the normally protective lactobacilli. BV is associated with an increased risk of acquiring sexually transmitted infections, including HIV, as well as with pregnancy complications. Mycoplasma hominis Ureaplasma urealyticum. The ease with which Mycoplasma hominis can be recovered and the frequency of its occurrence in septicemia may not be fully appreciated. We detected the growth of M. hominis radiometrically with an automated blood culture instrument. The organism grew in both aerobic and anaerobic culture media, but the cultures were not visibly positive Facultatively anaerobic (except M. pneumoniae, which is a strict aerobe) • Mycoplasma pneumoniae (also called Eaton agent) - most important • Mycoplasma genitalium. Mycoplasma hominis. Ureaplasma urealyticum. P1 adhesin protein binds to base of cilia on epithelial cells, leading to eventual loss of ciliated epithelial cells • Stimulates.

Mycoplasma hominis (M. hominis). This clinical syndrome also has other names, namely Haemophilus vaginalis vaginitis, nonspecific vaginitis or Gardnerella namely aerobic and anaerobic bacteria. At the time BV appears, there is an overgrowth of some bacterial species which are normally present in lo A genus of nonmotile facultatively anaerobic bacteria that lack a true cell wall, are gram-negative, and require sterol for growth. Definition (NCI_CDISC) Any bacteria that is not assigned to the species level but can be assigned to the Mycoplasma genus level Toll-like receptor 2 for binding of Mycoplasma and activation of inflammatory mediators, including cytokines. M. pneumoniae grows under both aerobic and anaerobic conditions ,isolated on media supplemented with serum. The organism most commonly exists in a filamentous form and has adherence proteins that attach to epithelial membranes with. facultative anaerobic bacilli: Gardnerella vaginalis, Prevotella spp., Bacteroides spp., Mobiluncus spp., Gram positive cocci, and genital Mycoplasma (Mycoplasma hominis and Ureaplasma urealiticum). This overgrowth is caused by a decrease i

Mycoplasma hominis - Microbe Canva

Europe PMC is an archive of life sciences journal literature. The activities of several oxidoreductases were measured in three fermentative and two nonfermentative Mycoplasma species that were grown under aerobic or anaerobic conditions Mycoplasma hominis, only metronidazole treatment resulted in a significant decrease in the frequency and concentration of Prevotella bivia and black-pigmented Prevotella species. Of the 865 anaerobic gram-negative rods evaluated for susceptibility, only 3 (0.3%) were resistant to metronidazole, whereas clindamyci

Mycoplasma hominis

Mycoplasma hominis Method 58 Mycoplasma pneumoniae Method 59 Myroides species Method 2 Atmosphere Aerobic Growth Time 24 to 48 hours . TIB.081 Revision K Page 7 of 21 Growth Time 24 to 48 hours Method 4 Media Anaerobic Blood Agar Temperature 35°C Atmosphere Anaerobic Growth Time 48 to 72 hours Note: Some obligate anaerobes may require. Bacterial Pathogen Pronunciation Station. It is difficult for students not familiar with the names of bacteria to pronounce them. This sound station contains links to sound files of these bacteria. There are other ways to pronounce these bacteria. This is the way I pronounce them when teaching our medical students at ATSU/KCOM*

A controlled study of genital mycoplasmas in amniotic

Lack of association between Mycoplasma hominis and

No single aerobic or anaerobic organism could be related to abnormal semen samples. Only the presence of U. urealyticum correlated significantly with abnormal semen samples (P less than 0.005). The most affected parameters were the number of spermatozoa (P less than 0.005) and motility (P less than 0.05) Recent studies suggested that interactions between various organisms, including GABHS, other aerobic and anaerobic bacteria, and viruses, may occur during PT. Some of these interactions may be synergistic (i.e., between EBV and anaerobic bacteria), thus enhancing the virulence of some pathogens, whereas others may be antagonistic (i.e., between. M. hominis has been isolated in 35-8% of patients and in 23.8% of healthy women. Research results of bacterial flora, aerobic and anaerobic, did not appear very different in two groups of women studied, with respect to presence or not of Mycoplasma. Among.. No acid-fast bacilli or other bacteria were observed on blood and tracheal aspirate smears. In addition, thoracic radiography showed only bilateral diffuse pulmonary infiltrates, which was not suggestive of an anaerobic infection. On day 2 of hospital admission, blood and throat samples were cultured for aerobic flora and mycoplasma Hematoma infection with Mycoplasma hominis following transplant nephrectomy pin-point colonies were noted on both aerobic and anaerobic blood agar, but no growth on McConkey's agar.These colo-nies, which failed to stain by Gram's method, were identi¢ed as M. hominis. Antibiotic treatment was therefore changed t

Proteome analysis of Mycoplasma fermentans cultured under

Mycoplasmosis in Dogs. Mycoplasmosis is the general medical name given to a disease caused by any one of three infectious agents: mycoplasma, t-mycoplasma or ureaplasma, and acholeplasma. Each of these agents is an anaerobic bacterial parasitic microorganism. They are capable of living and growing even without the presence of oxygen (anaerobic. consisting of an aerobic and an an-aerobic bottle (Bactec 9000, Becton Dickinson and Co, Franklin Lakes, Aerobic and anaerobic cultures of the excised mi-tral valve tissue tested negative. Mycoplasma hominis endocardi-tis in a child with a complex congenital heart defect. Pediatr Infect Dis J. 200 consisted of a semiquantitative aerobic culture, an anaerobic culture, and a culture for the genital mycoplasmas, Ureaplasma urealyticum and My­ coplasma hominis. For aerobic cultures, one drop of semen (± 0.05 mn was inoculated onto blood agar, blood agar with 0.004% nalidixic acid, Mac­ Conkey agar (GIBCO Europe, Paisley, Scotland)

Mycoplasma hominis〔なにこれ見えない〕 | グラム染色: Gram Stain

Mycoplasma hominis was cultured in 10 (2%) of 555 episodes of ACS and occurred more frequently in older patients, but the presenting symptoms and clinical course was similar to those with M pneumoniae. Conclusions. M pneumoniae is commonly associated with the ACS in patients with sickle cell anemia and occurs in very young children. M hominis. The Mycoplasma Laboratory at FDA had evaluated the relative recovery of cell culture contaminants using anaerobic vs. aerobic conditions and found that increased recovery of cell culture contaminants using both aerobic and anaerobic incubation was minimal compared to anaerobic incubation alone, as also seen in other laboratories [38-40] > 72 h @ 36 ± 1°C, aerobic 3. Biological control Inoculum size for productivity: 10 - 100 cfu Inoculum size for selectivity: 410 - 105 cfu Inoculum size for specificity: < 10 000 cfu Incubation conditions: 48 h @ 36 ± 1°C, anaerobic Control Strain Growth Mycoplasma hominis ATCC ® 14027™ Typical fried-egg colonies

Mycoplasma hominis / Ureaplasma urealyticum

MYCOPLASMA AND UREAPLASMA - microbiologybook

These are often due to chronic infections by Mycoplasma spp. (48), Chlamydia spp. (49) and possibly other infectious agents. Mycoplasmal infections are also associated with a variety of illnesses, such as M. hominis infections in patients with hypogammaglobulinemia (29), and M. genitalium with nongonococcal urethritis (50) Use for aerobic and anaerobic bacterial culture and fungal culture. Use double swab when both culture and M hominis, Ureaplasma, M genitalium, and/or bacterial vaginosis (BV) from vaginal fluid specimens. Mycoplasma and Ureaplasma culture, b) M4 may be kep Anaerobic / aerobic blood culture bottles are the only acceptable collection devices for blood. Ureaplasma / Mycoplasma identification / confirmation, with or without presumptive species indicated Reference Bacteriology Requisitio Aerobic and Anaerobic Bacterial Flora in Semen from Fertile and Infertile Groups of Men. Corynebacterium species, Mycoplasma hominis, and Ureaplasma urealyticum were isolated from group A. Group B revealed these organisma plus Streptococcus pneumoniae type III, Strep, pyogenes group A, Strep, feacalis, Escherichia coli,. The specimens obtained after prostatic massage of the remaining 26 patients were completely negative for both aerobic and anaerobic bacteria. No Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum or Trichomonas vaginalis were isolated from these patients. Patients with chronic prostatitis who gave positive culture results for.

Mycoplasma hominis is a common inhabitant of the vagina and can cause infections of the female and male genital tracts. Mycoplasma pneumoniae can infect the upper respiratory tract and the lungs. It is a major cause of respiratory infection in children of school age and young adults. A genus of aerobic to facultatively anaerobic bacteria. MHRP / Mycoplasma hominis, Molecular Detection, PCR, Varies Positive Positive Negative Negative Directed testing based on histopathology findings Acute inflammation ±microorganisms No evidence of inflammation or microorganisms Bacterial Culture, Aerobic and Anaerobic wit Mycoplasma ( C0026934 ) A genus of nonmotile facultatively anaerobic bacteria that lack a true cell wall, are gram-negative, and require sterol for growth. Any bacteria that is not assigned to the species level but can be assigned to the Mycoplasma genus level. A genus of gram-negative, mostly facultatively anaerobic bacteria in the family. Ureaplasma urealyticum is a bacterium that is found in the urogenital tracts of humans. It stains gram negative, but that is because it lacks a cell wall. This organism can exist as normal commensal flora in the reproductive tract (especially in women) and remain undetected unless specifically tested for 3 In recent years, the capabilities of real-time PCR - one of the most precise and advanced methods - have widened significantly: apart from identification of microorganisms, th

Objective: The involvement of the genital mycoplasmas Ureaplasma urealyticum and Mycoplasma hominis in complications of pregnancy has remained controversial especially because these microorganisms are frequent colonizers of the lower genital tract. Recovery of bacteria from the placenta appears to be the sole technique to represent a true infection and not vaginal contamination Mycoplasma Frequency Metabolism Atmosphere Ureaplasma urealyticum common Urea anaerobic Mycoplasma hominis common Arginine aerobic Mycoplasma fermentans occasionally Glucose and arginine anaerobic Mycoplasma genitalium common Glucose anaerobic Mycoplasma penetrans occasionally Glucose and arginine anaerobic Ureaplasma urealyticum was previously. Mycoplasma Agar Base (PPLO Agar Base) the Mycoplasma species are aerobic or facultatively anaerobic but some are microaerophilic. Few are anaerobic saprophytic Mycoplasma which grow best at 22-35°C while pathogenic strains grow at 35°C. Mycoplasma when grow in the agar medium show typical morphology and form colonies below the agar

Ureaplasma urealyticum and Mycoplasma hominis have been isolated from placental membranes in 47% and 30% of causes of confirmed chorioamnionitis, respectively. These organisms are traditionally viewed as commensal organisms in the lower genital tract with low virulence for infection. Swabs for aerobic and anaerobic placental cultures were. The aerobic bacteria also found mixed with these anaerobic bacteria include Enterobacteriaceae, Streptococcus spp. (including groups A and B), Neisseria gonnorhoeae and Chlamydia spp. and Mycoplasma hominis. Free gas in the tissues, abscess formation and foul-smelling discharge is commonly associated with the presence of anaerobic bacteria Urinary tract infections (UTIs) include infections of the urethra, bladder, and kidneys, and are common causes of urethritis, cystitis, pyelonephritis, and glomerulonephritis. Bacteria are the most common causes of UTIs, especially in the urethra and bladder. Cystitis. Cystitis is most often caused by a bacterial infection of the bladder, but it can also occur as a reaction to certain. The conjunctiva of newborns acquires facultative and anaerobic bacteria during birth primarily from the mother's cervical flora during passage through the birth canal (14). The role of anaerobes in neonatal conjunctivitis was investigated by obtaining conjunctival cultures from 35 babies prior to silver nitrate application and 48 hours later (19)

Mycoplasma definition of Mycoplasma by Medical dictionar

Mycoplasma hominis. A common inhabitant of the vagina and cervix and a potential human pathogen, causing infections of the male and female reproductive tracts. It has also been associated with respiratory disease and pharyngitis. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the. Mycoplasma hominis 4 % Coagulase + staphylococcus < 5 % (S. aureus Coagulase - Staphylococci ≥ % % Anaerobes (cteroides, Prevotella, Mobiluncus % Group B streptococci ≈ Gardnerella vaginalis Anaerobic streptococci ≥ % Ureaplasma spp % Enterobacteriaceae (% E. coli, Proteus, etc. % Candida albicans % Enterococci ≈ roupable Streptococci.

Ureaplasma Infections

Aerobic Gram-Positive Rods, Spirochetes, Mycoplasmas and

MYCOPLASMAS Characteristics n Lacking cell wall n Antimicrobial Susceptibility Panel, Anaerobic Bacteria, MIC (Gradient) - Resistance of anaerobic organisms to antibiotics has been increasing in recent years. Significant increases in resistance are now seen with non-bacteroides fragilis group gram-negatives and other genera such as clostridium, fusobacterium, prevotella, and peptostreptococcus Other species that reside in genital tract i.e, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, are the cause of 20% cases of non-gonococcal urithritis, post-pregnancy pelvic inflammatory disease (PID) They can survive in aerobic as well as in anaerobic condition Intraabdominal abscess formation occurs in 5% to 15% of patients with acute salpingitis as a result of a mixed aerobic and anaerobic infection. Abscess can occur within the tube, within the ovary, or between genital and gastrointestinal structures. Classic genital Mycoplasma may not cause primary tubal infection a.Neisseria gonorrhoeae b.Treponema pallidum c.Haemophilus ducreyi d.Chlamydia trachomatis e.Mycoplasma hominis. Aerobic and anaerobic blood cultures b) Stool culture c) DNA probe d) Urine culture e) Culture and dark field microscopy The correct answer is 'c'.

The possible role of anaerobic bacteria in chronic prostatiti

Conjunctivitis in the newborn infant usually is due to chemical and mechanical irritation caused by the instillation of silver nitrate drops or ointment into the eye in order to prevent gonorrheal ophthalmia. Chemical conjunctivitis differs from infective forms in that it becomes apparent almost immediately after the instillation. The most common causes of infectious conjunctivitis in. Cells cultured under anaerobic conditions demonstrated a high rate of recovery, that is, about 99·5% compared with titres determined before freezing (Fig. 4). When cells were cultured under aerobic conditions, however, titres were reduced by at least four logs when compared with initial titres (Fig. 4)

MycoplasmaMycoplasma hominis SP4 broth and agar with arginine added

Gram stain and culture for Mycoplasma hominis, Ureaplasma urealyticum, aerobic, and anaerobic bacteria were performed. Subjects were studied prospectively for the development of positive amniotic fluid cultures and the development of clinical chorioamnionitis. Results: The diagnosis of clinical chorioamnionitis was made in 25 % (15/60) of women. Mycoplasma Mycoplasma: The mycoplasma are a very large group of bacteria. There are more than 70 types. Mycoplasma hominis and Mycoplasma pneumoniae are among the dozen types of mycoplasma that occur in humans. Mycoplasma hominis is a common inhabitant of the vagina and can cause infections of the female and male genital tracts Mycoplasma contamination affects many different aspects of cell culturing, resulting in unreliable experimental results and potentially harmful biological products. Therefore, the specificity, sensitivity, and reliability of detecting mycoplasma contamination are important aspects of quality control in biotechnological products. In this study, Mycoplasma hyorhinis was adopted as a model strain.